Slicing and incubation mouse kidney slices |
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Materials |
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Solutions |
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Protocol |
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Note: Medium must be filled into plates, oxygenated in incubator for 30min-1h before incubate with slice.
ATP and RNA samples must be snap-frozen in liquid Nitrogen.3x1mL medium for each experiment condition will be collected into micronic-well.
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Remarks: |
Always keep tissue material cold and wet |